مقاله Molecular typing of Leishmania major parasite in the main vector of leishmaniasis in Khuzestan by targeting the ITSدرrDNA gene word دارای 2 صفحه می باشد و دارای تنظیمات در microsoft word می باشد و آماده پرینت یا چاپ است
فایل ورد مقاله Molecular typing of Leishmania major parasite in the main vector of leishmaniasis in Khuzestan by targeting the ITSدرrDNA gene word کاملا فرمت بندی و تنظیم شده در استاندارد دانشگاه و مراکز دولتی می باشد.
این پروژه توسط مرکز مرکز پروژه های دانشجویی آماده و تنظیم شده است
توجه : در صورت مشاهده بهم ریختگی احتمالی در متون زیر ،دلیل ان کپی کردن این مطالب از داخل فایل ورد می باشد و در فایل اصلی مقاله Molecular typing of Leishmania major parasite in the main vector of leishmaniasis in Khuzestan by targeting the ITSدرrDNA gene word ،به هیچ وجه بهم ریختگی وجود ندارد
بخشی از متن مقاله Molecular typing of Leishmania major parasite in the main vector of leishmaniasis in Khuzestan by targeting the ITSدرrDNA gene word :
سال انتشار: 1392
محل انتشار: هشتمین همایش بیوتکنولوژی جمهوری اسلامی ایران و چهارمین همایش ملی امنیت زیستی
تعداد صفحات: 2
نویسنده(ها):
Roozbeh Taslimian – Molecular Systematics Laboratory, Deparment of Parasitology, Pasteur Institute of Iran, Tehran.
Parviz Parvizi – Molecular Systematics Laboratory, Deparment of Parasitology, Pasteur Institute of Iran, Tehran.
Dr. Ahmad Reza Esmaili – Molecular Systematics Laboratory, Deparment of Parasitology, Pasteur Institute of Iran, Tehran.
Adel Spotin – Molecular Systematics Laboratory, Deparment of Parasitology, Pasteur Institute of Iran, Tehran
چکیده:
Background & Objective:Leishmania major is an underlying agent of zoonotic cutaneous leishmaniasis in an endemic focus of Khuzestan. In this study, we diagnose Leishmania parasites by targeting of ITS-rDNA gene because there is no molecular typing and characterization which has been carried out on P. papatasi as a main vector of L. major yetMaterials & Methods:Sandflies were caught using sticky traps and light traps. After dissecting, sandflies wereidentified. DNA was extracted and ITS-rDNA gene of Leishmania in sandflies amplified byPCR methods and by RFLP and sequencing then using molecular software Leishmania majorwas definitively approved.Results:Among 1500 sandflies, 198 female P. papatasi were selected as gravid, semi gravid and freshfed. DNA was extracted from all of them and the ITS-rDNA gene was amplified. About 8percent of sandflies was infected with Leishmania parasites. After RFLP and sequencing samples Leishmania major was identified in all cases.Conclusions:The sequences results obtained in this study compared with similar cases recorded in the GenBank and confirmed.
